TXN protein (His tag) (80R-1295)

Purified recombinant Human TXN protein

Synonyms FAD/NAD(P)-binding protein, TRX protein, ECK0879 protein, Thioredoxin JW0871 protein, TRX1 protein, Thioredoxin reductase protein, TRXR.
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction cocktail in a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant His-TXN into reaction cocktail and mix immediately. 3. Incubate at 25 DEG C for 20 minutes. 4. Record the increase in A650nm for 15 minutes

Images

Coomassie Blue stained SDS-PAGE of TXN protein (His tag) (80R-1295)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-105 amino acids: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 13.9 kDa (125aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of the sequestosome 1 protein commonly cause Paget?s disease since the UBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of TXN protein (His tag) (80R-1295) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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