Tymidylate synthase protein (His tag) (80R-1233)
Purified recombinant Human Tymidylate synthase protein
|Synonyms||Thymidylate synthetase protein, MGC88736 protein, Thymidylate synthase protein, EC 220.127.116.11 protein, d TMP synthase protein, TYMS protein protein, TS protein, , Tyms thymidylate synthetase protein, Tsase protein, HST422 protein, TYMS protein, TMS protein|
Coomassie Blue stained SDS-PAGE of Tymidylate synthase protein (His tag) (80R-1233)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-313 amino acids: MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV|
|Grade & Purity||> 95% pure|
|Molecular Weight||37.8 kDa (333aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1 M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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