Tyrosine Hydroxylase antibody (70R-51657)

Purified Polyclonal Tyrosine Hydroxylase antibody

Synonyms Polyclonal Tyrosine Hydroxylase antibody, Anti-Tyrosine Hydroxylase antibody, TYH antibody, Tyrosine 3-monooxygenase antibody, Tyrosine 3-hydroxylase antibody, TH antibody
Specificity Recognizes endogenous levels of Tyrosine Hydroxylase protein
Cross Reactivity Mouse, Rat, Bovine, Dog
Applications ICC, IF, IHC, IP, WB
Immunogen Tyrosine Hydroxylase antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Tyrosine Hydroxylase as the immunogen

Images

Western blot analysis using Tyrosine Hydroxylase antibody (70R-51657)

Western blot analysis of Tyrosine Hydroxylase expression in DLD (A); mouse liver (B); PC12 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Tyrosine Hydroxylase antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Tyrosine Hydroxylase catalyzes the reaction in which L-tyrosine is hydroxylated in the meta position to obtain L-3,4-dihydroxyphenylalanine (L-DOPA). The enzyme is an oxygenase which means it uses molecular oxygen to hydroxylate its substrates.

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Images

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Western blot analysis using Tyrosine Hydroxylase antibody (70R-51657)



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Western blot analysis of Tyrosine Hydroxylase expression in DLD (A); mouse liver (B); PC12 (C) whole cell lysates.
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Immunofluorescent analysis using Tyrosine Hydroxylase antibody (70R-51657)



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Immunofluorescent analysis of Tyrosine Hydroxylase staining in DLD cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis using Tyrosine Hydroxylase antibody (70R-51657)



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Immunohistochemical analysis of Tyrosine Hydroxylase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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