Recognizes endogenous levels of Tyrosine Hydroxylase protein
Cross Reactivity
Mouse, Rat, Bovine, Dog
Applications
ICC, IF, IHC, IP, WB
Immunogen
Tyrosine Hydroxylase antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Tyrosine Hydroxylase as the immunogen
Western blot analysis using Tyrosine Hydroxylase antibody (70R-51657)
Western blot analysis of Tyrosine Hydroxylase expression in DLD (A); mouse liver (B); PC12 (C) whole cell lysates.
Tyrosine Hydroxylase antibody
Western blot analysis using Tyrosine Hydroxylase antibody (70R-51657)
Western blot analysis of Tyrosine Hydroxylase expression in DLD (A); mouse liver (B); PC12 (C) whole cell lysates.
Immunofluorescent analysis using Tyrosine Hydroxylase antibody (70R-51657)
Immunofluorescent analysis of Tyrosine Hydroxylase staining in DLD cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis using Tyrosine Hydroxylase antibody (70R-51657)
Immunohistochemical analysis of Tyrosine Hydroxylase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
Tyrosine Hydroxylase antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Tyrosine Hydroxylase catalyzes the reaction in which L-tyrosine is hydroxylated in the meta position to obtain L-3,4-dihydroxyphenylalanine (L-DOPA). The enzyme is an oxygenase which means it uses molecular oxygen to hydroxylate its substrates.