UBE2B antibody (70R-12534)

Affinity purified Rabbit polyclonal UBE2B antibody

Synonyms Polyclonal UBE2B antibody, Anti-UBE2B antibody, UBEB-2, HHR6B antibody, UBEB-2 antibody, ubiquitin-conjugating enzyme E2-17 kDa antibody, UBEB 2 antibody, HR6B antibody, ubiquitin-protein ligase B antibody, ubiquitin-conjugating enzyme E2B (RAD6 homolog) antibody, UBE2B, ubiquitin-conjugating enzyme E2 B antibody, UBE2B antibody, ubiquitin carrier protein B antibody, RAD6 homolog B antibody, UBEB 2, UBC2 antibody, E2-17kDa antibody, RAD6B antibody
Cross Reactivity Human, Mouse
Applications WB
Immunogen UBE2B antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 182 of UBE2B

Images

Western blot analysis using UBE2B antibody (70R-12534)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 12 % SDS PAGE gel and UBE2B antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification UBE2B antibody was purified by antigen-affinity chromatography
Molecular Weight 17 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.71 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using UBE2B antibody (70R-12534)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 12 % SDS PAGE gel and UBE2B antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Western blot analysis using UBE2B antibody (70R-12534)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 12 % SDS PAGE gel and UBE2B antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using UBE2B antibody (70R-12534)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B antibody at 1:500 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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