UBE2L6 antibody (10R-1106)

Mouse monoclonal UBE2L6 antibody

Synonyms Monoclonal UBE2L6 antibody, Anti-UBE2L6 antibody, Ubiquitin conjugating enzyme E2L 6 MGC40331 antibody, UBEL6 2 antibody, Ubiquitin/ISG15 conjugating enzyme E2 L6. antibody, RIG B antibody, UBEL6 2, Retinoic acid induced gene B protein antibody, UBE2L6, Ubiquitin protein ligase L6 antibody, UBE2L6 antibody, RIG-B antibody, Ubiquitin carrier protein L6 antibody, Ubiquitin protein ligase antibody, Ubiquitin carrier protein antibody, UBEL6-2 antibody, Ubiquitin conjugating enzyme E2L 6 antibody, UBCH8 antibody, UBEL6-2, UBCH 8 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen UBE2L6 antibody was raised in mouse using recombinant human UBE2L6 (1-152aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of UBE2L6 antibody (10R-1106)

Cell lysates of MCF7 (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone k1H3
Isotype IgG2b kappa
Source Anti-human UBE2L6 antibody, clone k1H3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UBE2L6 protein.
Method of Purification UBE2L6 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:1,000-1:3,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

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Images

  • Western Blot analysis of UBE2L6 antibody (10R-1106) | Cell lysates of MCF7 (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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