UBE2S antibody (10R-1162)

Mouse monoclonal UBE2S antibody

Synonyms Monoclonal UBE2S antibody, Anti-UBE2S antibody, UBES-2 antibody, E2-EPF antibody, E2EPF antibody, UBES-2, Ubiquitin carrier protein S antibody, EPF5 antibody, UBES 2 antibody, Ubiquitin conjugating enzyme E2S antibody, Ubiquitin protein ligase S antibody, UBE2S, UBE2S antibody, Ubiquitin conjugating enzyme E2 24 kD antibody, MGC101982 antibody, UBES 2, Ubiquitin-conjugating enzyme E2S AA409170 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen UBE2S antibody was raised in mouse using recombinant human UBE2S (1-222aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of UBE2S antibody (10R-1162)

Cell lysates of 293T (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2C12
Isotype IgG2a kappa
Source Anti-human UBE2S antibody, clone AT2C12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UBE2S protein.
Method of Purification UBE2S antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

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Images

  • Western Blot analysis of UBE2S antibody (10R-1162) | Cell lysates of 293T (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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