UFC1 protein (His tag) (80R-1820)
Purified recombinant Human UFC1 protein
|Synonyms||Ubiquitin-fold modifier-conjugating enzyme 1 protein, UFC 1 protein, UFC 1, UFC-1, UFC-1 protein, UFC1, HSPC155 protein|
Coomassie Blue stained SDS-PAGE of UFC1 protein (His tag) (80R-1820)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-167 amino acids: MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ|
|Grade & Purity||> 90% pure|
|Molecular Weight||21.6 kDa (187aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. UFM1 is activated by a novel E1-like enzyme, UBA5, by forming a high-energy thioester bond. Activated UFM1 is then transferred to its cognate E2-like enzyme, UFC1, in a similar thioester linkage. Recombinant human UFC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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