Immunofluorescent staining using UNC13B antibody (70R-13408)
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using UNC13B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
UNC13B antibody
Immunofluorescent staining using UNC13B antibody (70R-13408)
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using UNC13B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunohistochemical staining using UNC13B antibody (70R-13408)
Immunohistochemical staining of paraffin-embedded A549 xenograft using UNC13B antibody at a dilution of 1:500
Western blot analysis using UNC13B antibody (70R-13408)
Western blot analysis of 30 ug of whole cell lysate (A: 293T) using a 5 % SDS PAGE gel and UNC13B antibody at a dilution of 1:500
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
UNC13B antibody was purified by antigen-affinity chromatography
Molecular Weight
181 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.