UNG protein (His tag) (80R-3498)
Purified recombinant UNG protein (His tag)
Overview
Overview
| Synonyms | ECK2578 protein, JW2564 protein |
|---|---|
| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE of ung protein (His tag) (80R-3498)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-229 aa: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYHGPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHREGVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Method of Purification | UNG protein was purified by using conventional chromatography techniques |
| Molecular Weight | 28.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol |
| Concentration | 500 ug/ml |
Storage & Safety
| Storage | Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | UNG, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. |
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