UNG protein (His tag) (80R-3498)

Purified recombinant UNG protein (His tag)

Synonyms ECK2578 protein, JW2564 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie blue stained SDS-PAGE of ung protein (His tag) (80R-3498)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-229 aa: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYHGPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHREGVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
Expression System E.coli
Grade & Purity > 90% pure
Method of Purification UNG protein was purified by using conventional chromatography techniques
Molecular Weight 28.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Concentration 500 ug/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance UNG, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps.

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Images

  • Coomassie blue stained SDS-PAGE of ung protein (His tag) (80R-3498) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €298.57
Size: 50 ug
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