VAMP4 protein (His tag) (80R-1178)
Purified recombinant Human VAMP4 protein
|Synonyms||VAMP24 protein, VAMP4 protein protein, VAMP-4 protein, VAMP4, VAMP 4, Vesicle associated membrane protein 4 protein, isoform CRA a protein, VAMP 4 protein, isoform CRA b., VAMP 4 protein, Vesicle-associated membrane protein 4 VAMP 24 protein, VAMP-4, Vesicle associated membrane protein 4 protein, Vesicle associated membrane protein 4 protein|
Coomassie Blue stained SDS-PAGE of VAMP4 protein (His tag) (80R-1178)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-115 amino acids: MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH|
|Grade & Purity||> 90% pure|
|Molecular Weight||14.5 kDa (123aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.1M NaCl, and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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