VAPA protein (His tag) (80R-1249)
Purified recombinant Human VAPA protein
Overview
Overview
| Synonyms | hVAP-33 protein, VAP 33 protein, VAMP associated protein A protein, VAP A protein, VAP-33 protein, VAP-A protein, Vesicle-associated membrane protein-associated protein A VAMP A protein, VAP33 protein, Vesicle associated membrane protein associated protein A. |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of VAPA protein (His tag) (80R-1249)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-227 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAS ASGAMAKHEQ ILVLDPPTDL KFKGPFTDVV TTNLKLRNPS DRKVCFKVKT TAPRRYCVRP NSGIIDPGST VTVSVMLQPF DYDPNEKSKH KFMVQTIFAP PNTSDMEAVW KEAKPDELMD SKLRCVFEMP NENDKLNDME PSKAVPLNAS KQDGPMPKPH SVSLNDTETR KLMEECKRLQ GEMMKLSEEN RHLRDEGLRL RKVAHSDKPG STSTASFRDN VTSP |
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| Expression System | E.coli |
| Grade & Purity | > 85% pure |
| Molecular Weight | 29.8 kDa (264aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein is a SNARE regulator with high levels of expression in the intestine during late embryogenesis and early neonatal development. VAPA may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Recombinant VAPA protein was expressed in E. coli and purified by using conventional chromatography techniques. |
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