Vaspin protein (His tag) (80R-1027)
Purified recombinant Human Vaspin protein
|Synonyms||OL64 OL 64 protein, Visceral adipose tissue-derived serine protease inhibitor protein, Serpin A12 precursor protein, serpin peptidase inhibitor clade A member 12 protein, ENSG00000165953 protein, OL-64 protein, SERPINA12 protein, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody|
Coomassie Blue stained SDS-PAGE of Vaspin protein (His tag) (80R-1027)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||21-414 amino acids: MGSSHHHHHH SSGLVPRGSH MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK|
|Grade & Purity||> 90% pure|
|Molecular Weight||47 kDa (415 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 8.0, containing 0.2mM PMSF, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques|
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