VCP antibody (70R-13516)

Affinity purified Rabbit polyclonal VCP antibody

Synonyms Polyclonal VCP antibody, Anti-VCP antibody, MGC131997 antibody, MGC148092 antibody, 15S Mg(2+)-ATPase p97 subunit antibody, IBMPFD antibody, VCP antibody, p97 antibody, MGC8560 antibody, TER ATPase antibody, transitional endoplasmic reticulum ATPase antibody, TERA antibody, yeast Cdc48p homolog antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB

Images

Western blot analysis using VCP antibody (70R-13516)

Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:5000

Specifications

Host Rabbit
Isotype IgG
Method of Purification VCP antibody was purified by antigen-affinity chromatography
Molecular Weight 89 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:1000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using VCP antibody (70R-13516)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunohistochemical staining using VCP antibody (70R-13516)</font></div>     | Immunohistochemical staining of paraffin-embedded Ca922 xenograft using VCP antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using VCP antibody (70R-13516)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: Hep G2; C: Molt-4; D: Raji) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using VCP antibody (70R-13516)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97) antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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