Western blot analysis using VCP antibody (70R-13516)
Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:5000
VCP antibody
Western blot analysis using VCP antibody (70R-13516)
Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:5000
Immunohistochemical staining using VCP antibody (70R-13516)
Immunohistochemical staining of paraffin-embedded Ca922 xenograft using VCP antibody at a dilution of 1:500
Western blot analysis using VCP antibody (70R-13516)
Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: Hep G2; C: Molt-4; D: Raji) using a 7.5 % SDS PAGE gel and VCP antibody at a dilution of 1:1000
Immunofluorescent staining using VCP antibody (70R-13516)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97) antibody at 1:200 dilution.
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
VCP antibody was purified by antigen-affinity chromatography
Molecular Weight
89 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.