VEGF antibody (70R-12327)
Rabbit polyclonal VEGF antibody
Overview
Overview
| Synonyms | Polyclonal VEGF antibody, anti-VEGF antibody, Vascular endothelial growth factor antibody |
|---|---|
| Cross Reactivity | Human |
| Applications | Neutralization, WB |
| Immunogen | VEGF antibody was raised in Rabbit using E coli expressed recombinant human VEGF as the immunogen. |
Images
Western Blot analysis using VEGF antibody (70R-12327)
Western blot analysis with recombinant his-tag VEGF protein. Lane 1: 10ng rh-VEGF. Lane 2: 50ng rh-VEGF. Lane 3: 250ng rh-VEGF. Lane 4: 1 ug rh-VEGF.
Specifications
| Host | Rabbit |
|---|---|
| Isotype | IgG |
| Method of Purification | VEGF antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer, pH 7.2, containing 30% glycerol and 0.01% thimerosal |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 2-3 ug/ml, Neutralization: 5-10 ug/ml, WB: 0.5-1 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | VEGF is a secreted homodimeric protein, secreted by a variety of vascularized tissues. VEGF stimulates endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. |
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