VEGF121 protein (30R-1282)

Purified recombinant Human VEGF121 protein

Synonyms VEGF-A protein, Vascular endothelial growth factor A protein, VPF protein, VEGF protein, VEGF A., Vascular permeability factor protein, Vascular endothelial growth factor A isoform f protein, Vascular endothelial growth factor A isoform f MVCD1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell Line: NIH-3T3 (Mus mosculus/ embryo) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 24 hr (after sample treatment) Concentration Range: 100 pg/ml - 10 ug/ml Detection method: BrdU assay

Images

Coomassie Blue stained SDS-PAGE of VEGF121 protein (30R-1282)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 207-327 amino acids: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 14.2 kDa (122aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of VEGF121 protein (30R-1282) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
OR
Shipping
View Our Distributors