VEGFB protein (His tag) (80R-1106)
Purified recombinant Human VEGFB protein
|Synonyms||Vascular endothelial growth factor B VEGF related factor protein, VEGFL protein, VRF protein, VEGF-related factor (VRF) protein, VEGFb|
Coomassie Blue stained SDS-PAGE of VEGFB protein (His tag) (80R-1106)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||22-207 amino acids: MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA|
|Grade & Purity||> 90% pure|
|Molecular Weight||21.6 kDa (207 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1 (VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is a homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. Recombinant VEGF-B protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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