VEGFR2 protein (Fc Chimera) (30R-AV017)
Purified recombinant Human VEGFR2 protein (Fc Chimera)
Overview
Overview
| Synonyms | VEGFR-2 protein, sKDR D1-7 protein, Protein-tyrosine kinase receptor Flk-1 protein, VEGFR-2 protein, CD309 protein, type III receptor tyrosine kinase protein, D1-7 proteinKDR D1-7 protein, FLK1 protein, KDR protein, Kinase insert domain receptor protein, VEGFR2-Fc Chimera |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Expression System | Baculovirus |
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| Grade & Purity | > 90% pure |
| Method of Purification | VEGFR2 protein (Fc Chimera) was purified by a proprietary chromatographic technique. |
| Molecular Weight | 160 kDa |
| Tag/Conjugate | Fc chimera |
| Form & Buffer | Lyophilized from a solution of 1x PBS, pH 7.4. Please Note: Buffer may change with new Lots. |
Usage & Assay Information
| Bioactivity | The activity of sVEGFR-2/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 10-30 ng/ml. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles. |
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General Information
| Biological Significance | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (kDaR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation.Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). |
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