Immunofluorescent staining using Villin antibody (70R-13325)
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Villin antibody
Immunofluorescent staining using Villin antibody (70R-13325)
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunohistochemical staining using Villin antibody (70R-13325)
Immunohistochemical staining of paraffin-embedded A549 xenograft using Villin antibody at a dilution of 1:500
Western blot analysis using Villin antibody (70R-13325)
Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5 % SDS PAGE gel and Villin antibody at a dilution of 1:5000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Villin antibody was purified by antigen-affinity chromatography
Molecular Weight
93 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.