Villin antibody (70R-13325)

Affinity purified Rabbit polyclonal Villin antibody

Synonyms Polyclonal Villin antibody, Anti-Villin antibody, VIL1 antibody, villin-1 antibody, D2S1471 antibody, villin 1 antibody, VIL antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using Villin antibody (70R-13325)

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Villin antibody was purified by antigen-affinity chromatography
Molecular Weight 93 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Villin antibody (70R-13325)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Villin antibody (70R-13325)</font></div>     | Immunohistochemical staining of paraffin-embedded A549 xenograft using Villin antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using Villin antibody (70R-13325)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5 % SDS PAGE gel and Villin antibody at a dilution of 1:5000

Availability: In stock

Price: €395.09
Size: 100 ul
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