virD2 protein (Agrobacterium tumefaciens) (His tag) (80R-3437)
Purified recombinant virD2 protein (Agrobacterium tumefaciens) (His tag)
Overview
Overview
| Synonyms | T DNA border endonuclease virD2 protein, vir D2 protein, vir-D2 protein |
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| Species | Agrobacterium tumefaciens |
| Protein Type | Recombinant |
| Applications | ELISA, SDS-PAGE, WB |
Specifications
| Expression System | E.coli |
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| Grade & Purity | > 90% pure |
| Molecular Weight | 56 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20mM Tris-Hcl, 0.5MNaCl, 10% glycerin (pH 8.0) and 200 mM Imidazole |
Storage & Safety
| Storage | Store at 4 deg C in a working aliquot for 1 week. For long term storage, aliquot and freeze at -20 to -80 deg C, avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | Tumor formation by A.tumefaciens involves the transfer and integration of a defined segment (T-DNA) of Ti plasmid DNA into the plant nuclear genome. virD2 operon encodes a site-specific endonuclease that cleaves at a unique site within both 24 bp direct repeats flanking the T-DNA. |
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