Wilms Tumor 1 antibody (70R-12541)

Affinity purified Rabbit polyclonal Wilms Tumor 1 antibody

Synonyms Polyclonal Wilms Tumor 1 antibody, Anti-Wilms Tumor 1 antibody, Wilms tumor 1 antibody, Wilms Tumor 1, WAGR antibody, GUD antibody, Wilms Tumor 1, WT33 antibody, AWT1 antibody, Wilms Tumor -1, NPHS4 antibody, WIT-2 antibody, amino-terminal domain of EWS|last three Zinc fingers of the DNA-binding domain of WT1 antibody, Wilms Tumor 1 antibody, Wilms Tumor -1 antibody, Wilms tumor protein antibody, WT1 antibody
Cross Reactivity Human, Rat
Applications ICC, IF, IHC-P, WB
Immunogen Wilms Tumor 1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 209 and 449 of Wilms Tumor 1

Images

Immunofluorescent staining using Wilms Tumor 1 antibody (70R-12541)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WT1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Wilms Tumor 1 antibody was purified by antigen-affinity chromatography
Molecular Weight 56 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.21 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Wilms Tumor 1 antibody (70R-12541)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WT1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Wilms Tumor 1 antibody (70R-12541)</font></div>     | Immunohistochemical staining of paraffin-embedded RT2 xenograft using Wilms Tumor 1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using Wilms Tumor 1 antibody (70R-12541)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Molt-4; B: Raji) using a 10 % SDS PAGE gel and Wilms Tumor 1 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
OR
Shipping
View Our Distributors