Wnt3a antibody (10R-1122)

Mouse monoclonal Wnt3a antibody

Synonyms Monoclonal Wnt3a antibody, Anti-Wnt3a antibody, Wnta-3, wingless-type MMTV integration site family antibody, Wnta-3 antibody, Wnta 3 antibody, Wnta 3, Wnt3a, member 3A antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen Wnt3a antibody was raised in mouse using recombinant human Wnt3a (19-352aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Wnt3a antibody (10R-1122)

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 3A6
Isotype IgG2a kappa
Source Anti-human Wnt3a antibody, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.
Method of Purification Wnt3a antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100. WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctional factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

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Images

  • Western Blot analysis of Wnt3a antibody (10R-1122) | The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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