Flow Cytometric assay using YARS antibody (10R-1196)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-YARS antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
YARS antibody
Flow Cytometric assay using YARS antibody (10R-1196)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-YARS antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
Western Blot analysis of immunized recombinant protein using YARS antibody (10R-1196)
Immunofluorescent staining using YARS antibody (10R-1196)
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-YARS antibody.
Specifications
Host
Mouse
Clone
YAR5H08
Isotype
IgG1
Method of Purification
YARS antibody was purified using protein G column chromatography from culture supernatant of hybridoma
cultured in a medium containing bovine IgG-depleted
(approximately 95%) fetal bovine serum.
Form & Buffer
YARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN )
as a preservative. There is a potential hazard that
NaN reacts with copper or lead to produce an explosive
compound. For safe disposal, the vial has to
be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.