YBX1 antibody (70R-14296)
Affinity purified Rabbit polyclonal YBX1 antibody
Overview
Overview
| Synonyms | Polyclonal YBX1 antibody, Anti-YBX1 antibody, YBX-1 antibody, Y box binding protein 1 antibody, YBX1, YB1 antibody, YBX 1 antibody, YBX-1, YBX 1, YB-1 antibody |
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| Applications | ICC, IHC-P, WB |
| Immunogen | YBX1 antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence in the middle regionof human CACYBP, identical to the related rat and Rabbit sequences, as the immunogen |
Images
Western Blot analysis using YBX1 antibody (70R-14296)
Lane 1:Rat Liver Tissue Lysate; Lane 2:Rat Brain Tissue Lysate; Lane 3:Rat Spleen Tissue Lysate; Lane 4:SMMC Cell Lysate; Lane 5: COLO320 Cell Lysate; Lane 6: SW620 Cell Lysate; Lane 7: 293T Cell Lysate
Specifications
| Host | Rabbit |
|---|---|
| Isotype | IgG |
| Method of Purification | YBX1 antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in lyophilized form |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by the end user |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage aliquot and store at -20 deg C, avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation. |
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