ZC3H8 antibody (70R-13054)

Affinity purified Rabbit polyclonal ZC3H8 antibody

Synonyms Polyclonal ZC3H8 antibody, Anti-ZC3H8 antibody, ZCH8 3, ZCH8-3 antibody, ZC3H8, Zinc finger CCCH-type containing 8 antibody, ZC3H8 antibody, ZC3HDC8 antibody, ZCH8 3 antibody, Fliz1 antibody, Zinc finger CCCH-type domain containing 8 antibody, ZCH8-3, Zinc finger CCCH domain-containing protein 8 antibody, Zinc finger CCCH type domain containing 8 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen ZC3H8 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 31 and 254 of ZC3H8

Images

Immunofluorescent staining using ZC3H8 antibody (70R-13054)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification ZC3H8 antibody was purified by antigen-affinity chromatography
Molecular Weight 34 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.84 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region of the GATA3 gene. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using ZC3H8 antibody (70R-13054)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using ZC3H8 antibody (70R-13054)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:A431; B:Hep G2; C:Raji) using a 12 % SDS PAGE gel and ZC3H8 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using ZC3H8 antibody (70R-13054)</font></div>     | Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using ZC3H8 antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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